Key attributes
SPECIFICATIONS
Tests per Box: 10
Catalog Number: MG-CA-R033
INTENDED USE
The Feline FPV/FHV/FCV Ab test is a quantitative assay used with an Immunofluorescence Analyzer to measure antibodies for feline panleukopenia virus (FPV), feline herpesvirus (FHV), and feline calicivirus (FCV) in serum or plasma.
TEST PRINCIPLE
This test uses a double antigen sandwich fluorescence method. The intensity of the fluorescent signal indicates the concentration of antibodies, expressed as Tu/ml.
MATERIALS
Provided:
10 sealed pouches with test devices for FPV-Ab, FHV-Ab, and FCV-Ab
Desiccant pouch
1 ID Chip
Instructions for Use
10 tubes of FPV/FHV/FCV Ab Sample Buffer
10 pipette tips
Required But Not Provided:
Immunofluorescence Analyzer
Timer
Pipette (already provided)
Centrifuge (typically not needed)
STORAGE AND STABILITY
Store at 4-30°C until the expiration date.
Perform tests at 18-28°C after opening.
Use the test within 30 minutes of opening the pouch.
SPECIMEN COLLECTION AND PREPARATION
Use serum or plasma (EDTA recommended).
Separate serum or plasma within 3 hours of collection. Use only clear, non-hemolyzed specimens.
Store specimens at 2-8°C for up to 72 hours or below -20°C for long-term storage.
TEST PROCEDURE
Preparation:
Place a Test Device on a flat surface.
Insert the ID Chip into the meter and ensure the Test Device lot number matches.
Sample Preparation:
Mix 20 μl of sample with FPV/FHV/FCV Ab Sample Buffer.
Run the Test:
Standard Test:
Pipette 100 μl of mixed sample into the test device.
Select "Standard Test" on the meter, insert the device, and click "Start Test." Repeat for each test device.
Quick Test:
Pipette 100 μl of mixed sample into the test device.
Select "Quick Test," start the timer, and let the device sit for 10 minutes. Insert it into the meter and click "Start Test." Repeat for each test device.
RESULTS
Results appear on the screen. Print results if needed.
QUALITY CONTROL
The test includes an internal quality control. If it fails, an error message will appear, and you'll need to use a new Test Device and sample.
INTERPRETATION
FPV-Ab: 4.5–640 Tu/ml
FHV-Ab: 5.0–640 Tu/ml
FCV-Ab: 5.0–640 Tu/ml
Refer to the results table for interpretation based on antibody levels.
WARNINGS AND LIMITATIONS
For in vitro diagnostic use only.
Do not use if packaging is damaged.
Use one pipette tip per specimen.
Results may be affected by technical errors or substances in the blood not listed.
Each lab should establish its own reference ranges.
